Journal: Cerebellum (London, England)

Article Title: Causal Evidence for a Role of Cerebellar Lobulus Simplex in Prefrontal-Hippocampal Interaction in Spatial Working Memory Decision-Making

doi: 10.1007/s12311-022-01383-7

Figure Lengend Snippet: Evaluation of plus-maze exploration behavior, spatial working memory, and classification of individual decisions. a Mice implanted with recording electrodes in the mPFC and dCA1 and an optical fiber above cerebellar LS were placed in a plus maze and allowed to freely explore for 12 min. Movement trajectories were video-captured and digitized for offline analysis. b The sequence of arm entries was analyzed to detect any sequence of four entries without a repeat (i.e., spontaneous alternations). In the example shown, arm entries that are part of a spontaneous alternation are printed in green font. A total of five spontaneous alternations are marked with grey brackets labeled SA1–5. Note that spontaneous alternations can be overlapping (SA2–5). The first occurrence of a spontaneous alternation in the example is a single sequence of four entries (CDAB, SA1). The second occurrence (CBADCBA) contains four overlapping spontaneous alternations (CBAD, BADC, ADCB, DCBA, SA2–5). c The same sequence as in (b) but with individual arm entry decisions classified as correct (green arrows) or incorrect (red arrows) based on the position of the decision in a sequence of four entries. For the analysis of decision-outcome–related neuronal activity only decisions that were preceded by three choices without repetition were classified as correct. As a consequence, the first two or three decisions of a spontaneous alternation (CBAD) were classified as incorrect depending on the choice immediately preceding the spontaneous alternation

Article Snippet: Electrophysiological recordings were conducted with extracellular recording electrodes (glass insulated tungsten/platinum; 80-μm diameter; impedance: 3.5–5.0 MΩ, Thomas Recording, GmbH, Germany) attached to a custom-made micro-drive.

Techniques: Sequencing, Labeling, Activity Assay

Journal: Neuron

Article Title: Cortical Output Is Gated by Horizontally Projecting Neurons in the Deep Layers

doi: 10.1016/j.neuron.2019.10.011

Figure Lengend Snippet:

Article Snippet: Extracellular multi-channel electrode recording amplifier , Intan Technologies , RHD2132.

Techniques: Recombinant, Plasmid Preparation, Software, Microscopy, Laser-Scanning Microscopy, Fluorescence